Studies of the cardiolipin interactome

CA Fox, RO Ryan - Progress in Lipid Research, 2022 - Elsevier
Gel filtration chromatography of the clarified sample revealed … and underwent a symptom
assessment evaluation. While … were observed in both symptom assessment scores and a 6-min …

Effects of phosphorylation on Drp1 activation by its receptors, actin, and cardiolipin

A Liu, AL Hatch, HN Higgs - Molecular Biology of the Cell, 2024 - Am Soc Cell Biol
… unilamellar vesicles containing 25 mole% cardiolipincardiolipin or the Drp1 receptors Mff
and MiD49. Drp1 phosphorylated in vitro on S579 displays similar attributes, with its cardiolipin

Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag

Z Wang, C Mim - Protein Expression and Purification, 2022 - Elsevier
… for structure determination. Here, we present an optimized … We used analytical gel filtration
to further characterize and … We added each protein to liposomes containing cardiolipin. We …

Formulation and Characterization of Bioactive Agent Containing Nanodisks

K Lethcoe, CA Fox, I Moh, M Swackhamer… - JoVE (Journal of …, 2023 - jove.com
… Characterization studies involve the determination of … liquid chromatography (FPLC) gel
filtration chromatography is … of an accessible cardiolipin bilayer 8 . Based on these, and other …

PB1F2 from influenza a virus regulates the interaction between cytochrome C and cardiolipin

Y Wang, J Wang - Membranes, 2022 - mdpi.com
… Based on this, we use two kinds of membrane mimic system … Finally, a Superdex 200 column
with gel filtration was used to … The determination of the MIP can provide information on the …

[PDF][PDF] Cryo-EM grid optimization for membrane proteins

D Kampjut, J Steiner, LA Sazanov - Iscience, 2021 - cell.com
… bottleneck in protein structure determination, especially for … by cation exchange
chromatography and gel filtration, while … proteins and 1:4 cardiolipin:dioleoyl phosphatidylcholine …

Ceramide enhances binding of LC3/GABARAP autophagy proteins to cardiolipin-containing membranes

YR Varela, MN Iriondo, A Etxaniz, U Ballesteros… - International Journal of …, 2022 - Elsevier
… its own specific application, and their combined use provides an … Plots in (E –J) can be
extrapolated to Δπ = 0 to estimate the … Finally, free Alexa Fluor 633 was removed by gel filtration in …

The Interplay among Subunit Composition, Cardiolipin Content, and Aggregation State of Bovine Heart Cytochrome c Oxidase

E Sedlák, T Kožár, A Musatov - Cells, 2020 - mdpi.com
… CcO, as measured by gel filtration, was often interpreted as … of mitochondrial CcO and the
determination of several crystal … is the result of the project implementation “Open scientific …

Determination of Gasdermin Pores

K Wang, J Ding, F Shao - NLR Proteins: Methods and Protocols, 2023 - Springer
… repeated concentration and dilution with the liposome wash buffer for at least ten cycles in
an Ultracentrifugal Filter. The Tb 3+ -encapsulated liposomes are then kept in Buffer L for use. …

[HTML][HTML] Bacillus subtilis cardiolipin protects its own membrane against surfactin-induced permeabilization

D Pinkas, R Fišer, P Kozlík, T Dolejšová… - … et Biophysica Acta (BBA …, 2020 - Elsevier
… Free probe was removed by gel filtration in a column (20 ml) filled with sephadex G50 (GE …
We decided to use a ternary system composed of those phospholipids present in the B. …